A comparison of the effectiveness of two disinfection methods for full dentures for patients treated at the Dr René Puig Bentz Dental Clinic at the National University of Pedro Henríquez Ureña.
Description
Full dentures fabricated from acrylic resin have been observed to encourage the accumulation of biofilm, a process which has been linked to a number of oral health complications. The present study sought to ascertain the comparative efficacy of 0.5% sodium hypochlorite and effervescent alkaline peroxide tablets in the disinfection of complete dentures for patients treated at the Dr. René Puig Bentz Dental Clinic. A cross-sectional experimental study was conducted with 35 dentures, which were divided into two groups based on the disinfection method used. The initial and post-treatment microbial loads were evaluated, and the present microorganisms were identified. The results demonstrated that sodium hypochlorite led to a substantial reduction in microbial load. In certain instances, the treatment has been shown to result in complete elimination of the condition, while in others, it has been observed to reduce the load by almost 99.9%. This phenomenon impacted various microorganisms, including Porphyromonas spp., Peptostreptococcus spp., yeasts, and spore-forming bacilli. Conversely, the bacterial load exhibited stability or increased with the utilisation of effervescent tablets, particularly in genera such as Streptococcus and yeast. Pursuant to the implementation of a statistical analysis, it was confirmed that there were significant discrepancies between the two methods (p < 0.05). This was determined by conducting a Fisher's F-test.
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The data were obtained using a cross-sectional experimental design in which 35 acrylic resin full dentures belonging to patients treated at the Dr. René Puig Bentz dental clinic were analyzed. Selection was based on previously defined inclusion criteria, considering dentures in active use and in conditions suitable for analysis. Subsequently, the samples were divided into two groups according to the disinfection protocol applied. To collect microbiological data, the sterile swabbing technique was used on the basal surface of each prosthesis, as this is the area of greatest contact with tissues and greatest biofilm accumulation. The swabs were inoculated into Trisoya broth (Tryptic Soy Broth) as a transport and enrichment medium. The samples were transported to the laboratory in insulated containers, maintaining a temperature between 2–8 °C to preserve microbial viability. In the laboratory, the samples were inoculated onto Petri dishes with culture media suitable for the growth of aerobic and anaerobic bacteria and yeasts commonly found on dental prostheses. The samples were incubated under controlled conditions of temperature (35–37 °C) and time (24–72 hours), allowing colony development. The identification of the microorganisms was carried out by evaluating macroscopic and microscopic characteristics, supplemented with biochemical tests when necessary.
Institutions
- Universidad Nacional Pedro Henríquez UreñaNacional, Santo Domingo