Western blot HADHA & HADHB deficient fibroblasts and controls
Published: 30 May 2023| Version 1 | DOI: 10.17632/dr4fhz7smm.1
Contributors:
Eduardo Vieira Neto, Description
Mitochondrial trifunctional protein (TFP) deficiency fibroblasts were submitted to sonication cell lysis. Western blots were performed with these cell lysates employing polyclonal anti-total TFP, monoclonal anti-HADHA, and monoclonal anti-HADHB antibodies, having TOMM-20 as a control.
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Mitochondrial trifunctional protein (TFP) α- and β-subunits and a protein tentatively attributed to monolysocardiolipin-acyltransferase (MLCLAT) were examined by SDS-PAGE and western blotting (WB).
Institutions
University of Pittsburgh
Categories
Inborn Error of Metabolism, Western Blot, Fatty Acid Oxidation
Funding
Children's Neuroscience Institute
2022-2023 INA Awardee