Gut microbiota remodeling by Astragalus membranaceus stems and leaves mediates dual modulation of arachidonic acid and tryptophan metabolism to counteract perinatal stress-induced hemometabolic dysregulation in sows
Description
Metabolomics preprocessed data, the data set is related to figures 6-9, Extended Data figures S4-11.
Files
Steps to reproduce
Lyophilized serum and fecal samples were thawed using an ice bath to minimize degradation. A 20-μL aliquot of serum sample or approximately 5 mg of fecal sample was then extracted with 120 μL of cold methanol/acetonitrile (1:1, v/v) and vortexed for 1 min. Following vortexing, the mixture was homogenized for 5 min and subsequently centrifuged at 12,000×g for 10 min at 4 °C. The supernatant was collected and 150 μL of an internal standard solution (2-chloro-l-phenylalanine at 4 ppm in 80% methanol/water) was added to resuspend the pellet. The supernatant was filtered through a 0.22 μm membrane and transferred into a detection vial for further analysis. Additionally, pooled quality control (QC) samples were prepared by combining 10 μL of each extract for assessment of method consistency. Metabolite profiling and data processing in serum samples were conducted using a Vanquish UHPLC system (Thermo Fisher Scientific, USA). Chromatographic separation was carried out with an ACQUITY UPLC-HSS T3 (2.1×100 mm, 1.8 μm) (Waters Corp., Milford, MA, USA) maintained at 40℃. Each sample was analyzed by UPLC-MS/MS in both positive and negative ionization modes to obtain the metabolite profiles. Mass spectrometric detection of metabolites was performed on an Orbitrap Exploris 120 mass spectrometer (Thermo Fisher Scientific, USA) equipped with an ESI ion source.
Institutions
- Beijing University of Agriculture