Metabolome data of rice rhizosphere soil after applying NH1 strain under cadmium stress
Description
Our study revealed that the application of Deinococcus sp. NH1 strain to rice seedlings under cadmium (Cd) stress effectively enhanced their Cd stress tolerance. To elucidate the mechanisms underlying the improved Cd resistance mediated by Deinococcus sp. NH1, we conducted a rigorously designed greenhouse pot experiment. The experiment comprised three treatment groups and one control group, as follows: Control group (CK): No Cd stress and Deinococcus sp. NH1 strain application; NH1 group (NH1): Application of Deinococcus sp. NH1 strain alone; Cd group (Cd): Application of CdCl₂ solution alone; Cd + NH1 group (Cd+NH1): Concurrent application of CdCl₂ solution and Deinococcus sp. NH1 strain. Rhizosphere soil samples were collected after a specified period and analyzed using liquid chromatography-mass spectrometry (LC-MS)-based untargeted metabolomics. The metabolomic data were acquired in both positive ion mode (POS) and negative ion mode (NEG), resulting in two separate folders labeled "POS" and "NEG" for downstream analysis.
Files
Steps to reproduce
In this study, rhizosphere soil samples of rice were collected from a total of four treatment groups, namely CK, NH1, Cd, and Cd + NH1. The sampling range was 1–2 mm around the roots. Six parallel samples were set for each treatment, resulting in a total of 24 samples. The detection work was entrusted to Guangdong Magigene Technology Co., Ltd. (https://www.magigene.com/). The specific experimental procedures and detection analysis steps are as follows: The collected rhizosphere soil samples were freeze - dried. Subsequently, under low - temperature conditions, 100 mg ± 1 mg of the samples were accurately weighed and placed in EP tubes. Then, 1000 μL of the extraction solution was added. After thorough treatment, the supernatant was taken for subsequent on - machine detection.Next, a Vanquish ultra - high - performance liquid chromatograph (Thermo Fisher Scientific) combined with a Phenomenex Kinetex C18 chromatographic column (2.1 mm × 50 mm, 2.6 μm) was used for the chromatographic separation of the target compounds. Mass spectrometry detection was performed using an Orbitrap mass spectrometer (Q Exactive Orbitrap, Thermo Fisher Scientific, USA). The acquisition of primary and secondary mass spectrometry data was completed under the regulation of the Xcalibur control software (version 4.4, Thermo).The off - machine mass spectrometry data were used for metabolite matching and identification through the DB database. Meanwhile, the internal standard method was employed to normalize the metabolite detection results to ensure the accuracy and comparability of the data.
Institutions
- Shandong Agricultural University