Western blots data about the protective effect of Rg3@PACVs in lung ischemia–reperfusion injury
Description
Rg3@PACVs + NIR treatment effectively preserved mitochondrial homeostasis and activated pro-survival signaling under IRI conditions. Specifically, IRI and H/R markedly downregulated PGC-1α and MFN2 while increasing DRP1 phosphorylation, indicating impaired mitochondrial biogenesis and fusion together with enhanced fission. In contrast, Rg3@PACVs + NIR significantly restored PGC-1α expression, upregulated MFN2, and inhibited DRP1 phosphorylation, thereby promoting mitochondrial biogenesis and fusion while suppressing excessive fission (Figures S3E and S5J). Moreover, Rg3@PACVs + NIR enhanced the phosphorylation of PI3K and AKT, suggesting activation of the PI3K/AKT pathway, which is crucial for the regulation of cell survival, proliferation, and metabolism (Figures S6G–H).
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Steps to reproduce
An automated capillary-based Western blotting system (Wes, ProteinSimple) was used to detect protein changes in nanoparticles, BEAS 2B cells, and rat lung tissue. Nanoparticle suspensions at a concentration of 1 mg/mL were lysed in RIPA lyse buffer (EZPS04-1, WSHTBio, Shanghai, China), and proteins were separated by molecular weight in the Wes system.