Growth Optimized Aminoacyl-tRNA Synthetase Levels Prevent Maximal tRNA Charging. Parker et al
Description
tRNA charging Northern blot to demonstrate increased charging of tRNA with cognate aaRS overproduction. One .tif file of multiple blots imaged together. Intensity was changed in figure to aid viewing. In order from left to right: Probed for argACG tRNA: Lane 1 - Deacylated control sample Lane 2 - WT 168 sample Lane 3 - ArgS overproduction sample Lane 4 - ArgS overproduction sample Replicate Lane 5 - SerS overproduction sample Probed for serGCU tRNA: Lane 1 - Deacylated control sample Lane 2 - WT 168 sample Lane 3 - SerS overproduction sample Lane 4 - IleS overproduction sample Probed for ileCAU tRNA: Lane 1 - Deacylated control sample Lane 2 - WT 168 sample Lane 3 - IleS overproduction sample Lane 4 - LeuS overproduction sample Probed for leuUAA tRNA: Lane 1 - Deacylated control sample Lane 2 - WT 168 sample Lane 3 - LeuS overproduction sample Lane 4 - IleS overproduction sample
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Steps to reproduce
See Methods section to reproduce protocol