Convolvulus pluricaulis confers antidepressant and antioxidant effects through conserved metabolic and molecular pathways in Drosophila Mitra et al.
Description
1. Figure 2A: Raw Data (Metabolomics)(Head Tissue) 2. Figure 2A: Metaboanalyst Input (Head Tissue) 3. Figure 2A: Normalized_Scaled_ C.p_Ctrl (Head Tissue) 4. Figure 2B: (PCA)(Head Tissue) 5. Figure 2C: (Volcano plot)(Head Tissue) 6. Figure S2A: (Raw Data) (Body w/o Head tissue) 7. Figure S2A: (MetaboAnalyst input) (Body w/o Head tissue) 8. Figure S2A: Normalized_Scaled_ C.p_Ctrl (Body w/o Head tissue) 9. Figure S2E: (PCA)(Body w/o Head tissue)
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Steps to reproduce
All GC-MS analysis was performed at the Core facility in the university of Utah with an Agilent 5977b GC-MS MSD-HES and an Agilent 7693A automatic liquid sampler. Dried samples were suspended in 40 µL of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for one hour at 37 °C in a sand bath. 25 µL of this solution was added to auto sampler vials. 60 µL of N-methyl-N-trimethylsilyltrifluoracetamide (MSTFA with 1%TMCS, Thermo #TS48913) was added automatically via the auto sampler and incubated for 30 minutes at 37 °C. After incubation, samples were vortexed and 1 µL of the prepared sample was injected into the gas chromatograph inlet in the split mode with the inlet temperature held at 250°C. A 5:1 split ratio was used for analysis for the majority of metabolites. Any metabolites that saturated the instrument at the 5:1 split were analyzed at a 50:1 split ratio. The gas chromatograph had an initial temperature of 60°C for one minute followed by a 10°C/min ramp to 325°C and a hold time of 10 minutes. A 30-meter Agilent Zorbax DB-5MS with 10 m Duraguard capillary column was employed for chromatographic separation. Helium was used as the carrier gas at a rate of 1 mL/min. Data was collected using MassHunter software (Agilent). Metabolites were identified and their peak area was recorded using MassHunter Quant. This data was transferred to an Excel spread sheet (Microsoft, Redmond WA). Metabolite identity was established using a combination of an in-house metabolite library developed using pure purchased standards, the NIST library and the Fiehn library. Data was analyzed using in-house software to prepare for analysis by the "MetaboAnalyst" software tool.
Institutions
- Shiv Nadar UniversityUttar Pradesh, Greater Noida