PAR2–β-arrestin 2–ERK axis mediates Malassezia globosa-induced IL-17 response by disrupting ZO-1 in keratinocytes-- original data
Description
Our upcoming article to be published in iScience, titled "PAR2–β-arrestin 2–ERK axis mediates Malassezia globosa-induced IL-17 response by disrupting ZO-1 in keratinocytes", presents our original, unprocessed data alongside our paper through Mendeley Data.
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The protein fractions were extracted using Protein Extraction Kit (Beyotime, Shanghai, China). Based on our previous experiment, the blots were incubated with primary antibodies PAR2, β-arrestin 2, p-ERK, ERK1/2, IL-17, ZO-1, Occludin, Claudin-1, and GAPDH at a 1:1000 dilution. They were then incubated with HRP-conjugated secondary antibodies (Proteintech, Wuhan, China) and visualized using enhanced chemiluminescence (Millipore, MA, USA). All experiments were conducted with three independent repetitions.
Institutions
- Affiliated Hospital of Guangdong Medical College HospitalGuangdong, Zhanjiang