A Conserved Antioxidant Defense at the Endoplasmic Reticulum Membrane, Zhijian Ji et al
Description
This dataset contains the original western blot images and microscopy data supporting the research article "A Conserved Antioxidant Defense at the Endoplasmic Reticulum Membrane" by Zhijian Ji et al. In this study, we combined AlphaFold2 and functional screens in C. elegans to discover ERGU-1, an evolutionarily conserved ER-transmembrane protein that supports ER proteostasis and provides antioxidant defense against hydrogen peroxide (H2O2). Microscopy Data: Epifluorescence and high-resolution confocal images demonstrating the subcellular localization of C. elegans ERGU-1 and its human homolog TMEM161B to reticular networks at the ER membrane. It also includes imaging data quantifying the activation of oxidative and ER stress reporters (e.g., gst-4p::GFP, hsp-4p::GFP) and ratiometric H2O2 sensors (roGFP2-Orp1). Western Blot Images: Original, uncropped blots demonstrating the redox-modulated oligomeric states of TMEM161B.