Resting state fMRI data from n=14 mice under medetomidine-isoflurane anesthesia (0.05 mg/kg bolus and 0.1 mg/kg/h IV infusion, plus 0.5% isoflurane) from Gutierrez-Barragan et al. 2021 (DOI pending). Scans were acquired at the IIT laboratory in Rovereto (Italy) on a 7.0 Tesla MRI scanner (Bruker Biospin, Ettlingen) with a BGA-9 gradient set, a 72 mm birdcage transmit coil, and a three-channel solenoid receive coil. Awake scans used a single-shot echo planar imaging (EPI) sequence with the following parameters: TR/TE=1000/15 ms, flip angle=60°, matrix=100 x 100, FOV=2.3 x 2.3 cm, 18 coronal slices (voxel-size 230 x 230 x 600 µm), slice thickness=600 µm and 1920 time points, for a total time of 32 minutes. It is suggested to remove the first 120 scans (2 minutes) to account for thermal gradient equilibration.
Steps to reproduce
Please follow the preprocessing and analysis methods in Gutierrez-Barragan et al., 2021 (DOI pending) and use the software listed in the KEY RESOURCES TABLE.