Pro-apoptotic activity of Apis mellifera royal jelly hydrophilic fractions

Published: 8 November 2022| Version 1 | DOI: 10.17632/fn95vr46kh.1
Contributor:
Ashutosh Acharya

Description

Apis mellifera royal jelly was separated into high and low molecular weight hydrophilic fractions and their antiproliferative activity against a highly metastatic human colorectal cancer cell line HCT-116 and a non-cancerous 3T3-L1 cell line was assayed using the MTT assay. The pro-apoptotic activity of the high molecular weight protein fraction was further assayed using various apoptotic markers and cell cycle analysis studies by flow cytometry.

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Categories

Flow Cytometry, Antiproliferative Activity, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, MTT Assay

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