Prognostic Stratification in Cervical Cancer

Published: 29 January 2026| Version 1 | DOI: 10.17632/fwftt7ndy5.1
Contributors:
Wassim Almawi,

Description

We genotyped nine functional SNPs across TNF-α (rs361525, rs1800629), *IL-1β* (rs16944), IFN-γ (rs2430561), *IL-1RN* (rs2234663), *IL-10* (rs3024490, rs1800872, rs1800871), and *IL-6* (rs1474348) in a cohort of 130 Tunisian CC patients. This focused, candidate-gene analysis identifies prognostic SNP-based subgroups and a nine-SNP polygenic risk score associated with survival in cervical cancer.

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Steps to reproduce

The selection of the studied SNPs was guided by evidence from previous case–control and genome-wide association studies reporting significant associations with CC risk. Genotyping was performed by the allelic (VIC and FAM-labelled) discrimination method. Assay-on-demand TaqMan assays were ordered from Applied Biosystems (Foster City, NJ). The reaction was performed in 6 μl volumes on StepOne/StepOne Plus real-time PCR systems, as recommended by the manufacturer (Applied Biosystems).

Institutions

  • Universite de Tunis El Manar Faculte des Sciences de Tunis
    Tunis

Categories

Cervical Cancer, Single Nucleotide Polymorphism

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