Combined Dataset Summary: Analysis of a Pink Pill Sample
Description
🔬 1. Microscopy: “Micrographs of pink pill” 📁 Dataset Link | DOI: 10.17632/c2xhxwddt6.1 Technique: Light microscopy (Carton) Magnification: 4× Purpose: Document the pill's external morphology Files: Image files showing shape, texture, and color Insights: Supports visual inspection and potential surface irregularities or embedded materials ⚗️ 2. GC-MS: “Analysis of pink pill by GCMS” 📁 Dataset Link | DOI: 10.17632/x7x7t4zfy7.1 Technique: Gas Chromatography–Mass Spectrometry Purpose: Identify organic compounds in solution Expected Findings: Methamphetamine Caffeine Phentermine Ethyl vanillin File Types: Likely includes chromatograms, mass spectra, and compound reports 📉 3. FTIR: “A scaled subtraction of ATR-FTIR spectra and spectra identification using Spectral Library Databases” 📁 Dataset Link | DOI: 10.17632/6dhrs9v9zf.1 Technique: ATR-FTIR (Attenuated Total Reflectance Fourier-Transform Infrared Spectroscopy) Workflow: Spectrum of pink pill acquired Reference (caffeine) spectrum subtracted using OPUS software Result matched against 3 IR libraries: General IR, SWGDRUG IR, Demo Library Purpose: To resolve overlapping spectral features and aid identification of multi-component samples Deliverables: Subtracted spectra, match hits from library search, and reproducible OPUS commands
Files
Steps to reproduce
🔬 1. Microscopy (Dataset: Micrographs of pink pill) Equipment Light microscope (e.g., Carton microscope) Camera (attached or digital eyepiece) Procedure Place the pink pill on a clean microscope stage without modifying or sectioning it. Capture images at 4× magnification using bright-field illumination. Record multiple images from different angles (if needed) to document morphology. Save images in standard formats (e.g., JPG or TIFF). ⚗️ 2. Gas Chromatography–Mass Spectrometry (Dataset: Analysis of pink pill by GCMS) Equipment & Software GC-MS instrument (e.g., Agilent or Thermo GCMS system) NIST library for compound identification Sample Preparation Dissolve a portion of the pink pill in a polar solvent (e.g., methanol or ethanol). Filter to remove particulates if needed. Inject a suitable volume (e.g., 1 µL) into the GC-MS. Instrument Conditions (general guidance) Column: DB-5MS or equivalent Carrier Gas: Helium Oven Program: Start at 60°C → ramp to 280°C MS Mode: Electron Ionization (EI), scan mode Data Analysis Analyze the total ion chromatogram (TIC). Compare mass spectra of peaks with the NIST MS Library. Record compound names, retention times, and match quality. 📉 3. ATR-FTIR Spectroscopy with Spectral Subtraction (Dataset: ATR-FTIR spectra subtraction) Equipment Bruker Alpha II or equivalent ATR-FTIR spectrometer OPUS software with spectral subtraction function Sample Preparation Place the intact or crushed pink pill directly on the ATR diamond crystal. Apply pressure clamp to ensure good contact. Collect spectra in the range 4000–400 cm⁻¹, 4 cm⁻¹ resolution, 16 scans. Reference Spectrum Obtain reference IR spectrum of caffeine anhydrous (from library or measured) Subtraction Process in OPUS Load the pink pill spectrum as the "sample." Load the caffeine spectrum as the "reference." Open the subtraction tool: Evaluate → Spectral Subtraction Scale the reference spectrum appropriately (e.g., manually or automatically using OPUS). Subtract and save the resulting spectrum. Spectral Matching Use the OPUS library search feature. Compare the subtracted spectrum against: General IR Library (350 spectra) SWGDRUG IR Library (503 spectra) Demo Library (200 spectra)
Institutions
- Universiti Putra Malaysia