Quantitative proteomics of inguinal white adipose tissue (iWAT) in young versus middle-aged male mice
Description
To deepen our understanding of the temporal dynamics of these metabolic changes, we conducted proteomic analyses of iWAT from both young and middle-aged mice. Our findings revealed that, compared with young mice, middle-aged mice presented a distinct pattern of differentially expressed proteins, which were associated mainly with adipose metabolism and mitochondrial function. These data suggest that age-related changes in protein expression are pivotal in the metabolic alterations observed in middle-aged mice. It is possible that GSNOR KO mice mitigate age-related metabolic decline by altering the expression patterns of these critical proteins.
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Steps to reproduce
We conducted a TMT-based quantitative proteomics analysis to compare the protein profiles of inguinal white adipose tissue (iWAT) between young and middle-aged mice. Proteins extracted from iWAT samples of middle-aged mice were digested into peptides and labeled with the 129, 130, and 131 TMT tags, while peptides from young mouse iWAT were labeled with the 126, 127, and 128 tags, respectively. The six labeled samples were then pooled into a single multiplexed sample, which was fractionated to reduce complexity. All fractions were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Following data acquisition, peptide identification and relative quantification were achieved by measuring the intensities of the reporter ions released from the TMT tags upon fragmentation, allowing for a direct comparison of protein abundance between the two age groups.
Institutions
- Institute of Biophysics Chinese Academy of Sciences