Quantifying yeast lipidomics by high performance thin layer chromatography (HPTLC) and comparison to mass spectrometry-based shotgun lipidomics.

Published: 21 February 2024| Version 1 | DOI: 10.17632/gf9z9ky3h5.1
Contributors:
, Oskar Knittelfelder,
,

Description

Here we present evidence that high performance thin layer chromatography (HPTLC) represents an effective alternative to replace cost intensive mass spectrometry-based lipidomic analyses. We provide statistical comparison of identical samples by both methods, which support the use of HPTLC for quantitative analysis of the main yeast lipid classes.

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Institutions

Universitat Witten/Herdecke

Categories

Lipidomics

Funding

Ministerium für Kultur und Wissenschaft des Landes Nordrhein-Westfalen

NRW-Rückkehr Programm - Patrick Rockenfeller

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