Data for Figures 2 B, C, D
Description
A probabilistic relationship between cell fate and cell cycle position B. Dose responsive induction and repression of prestalk and prespore marker gene expression. AX3 cells transformed with pspA-RFP, ecmAO-RFP or ecmB-RFP cell type specific reporters were incubated with indicated DIF-1 concentrations and number of expressing cells determined by FACS. C. Cell type differentiation at 10nM DIF-1. AX3 cells co-transformed with pspA, ecmAO or ecmB cell type specific reporters were incubated with 10nM DIF-1 and the number of expressing cells determined by FACS. D. Cell type differentiation is cell cycle dependent. 448 cells were tracked during growth and differentiation. Percentages of cells that differentiated as prespore or prestalk cells (ecmAO or ecmB expressing) were calculated for cells dividing within each hourly time intervals before induction. The likely cell cycle position is highlighted.