ORO staining of brains following clearing protocols

Description

We wanted to assess lipid content in cryosections, in control brains and following clearing treatments. We show raw images of ORO-stained sections, arranged in folders by the clearing treatment. An excel spreadsheet show the calculated intensity of each image, normalized to control group per experiment. Representative images presented in Data in Brief are all from experiment #1. Cryosections (mounted on microscope slides) were immersed in 0.3 % ORO in 60 % isopropanol (cat #: O0625, Sigma-Aldrich) and incubated 12 hrs at RT, then washed three times in PBS.

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