BOLD TaxonID Tree of water mites from the Balkan peninsula (BOLD Dataset “DS-BALHYD”)
Description
The Neighbour-Joining tree of all water mite (Acari, Hydrachnidia) species from the Balkan peninsula based on Kimura 2-parameter distances. The analyses involved 1026 COI nucleotide sequences publicly available through the Barcode of Life Data Systems (BOLD Dataset: https://portal.boldsystems.org/recordset/DS-BALHYD).
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Steps to reproduce
Part of the molecular analysis was conducted at the Institute of Biology, University of Szczecin (IoB-UoS), the Canadian Centre for DNA Barcoding (Guelph, Ontario, Canada (CCDB; http://ccdb.ca/)), the University of Florence (UNIFI), Florence, Italy, and at the University of Lodz (UniLodz), Lodz, Poland. DNA was extracted using a non-destructive protocol as described in Pešić et al. (2025b). At UniLodz amplification, and DNA sequencing using Oxford Nanopore Technology was done according to the protocol in Pešić et al. (2025b). Raw reads were demultiplexed using the Pacific Biosciences SMRT Link software. Consensus sequences were generated with the PacBio Amplicon Analysis (pbaa) tool. Primer trimming, translation and stop codon checking were performed using Geneious Prime 2024.0.1. Consensus sequences were made available in the BOLD database (Ratnasingham & Hebert 2007), and the Barcode Index Numbers (BIN) for every sequence were retrieved and analyzed. Relevant voucher information, photos, and newly generated DNA barcodes are publicly accessible through the Dataset “DS-BALHYD DNA barcode reference library of Balkans water mites”(https://portal.boldsystems.org/recordset/DS-BALHYD).
Institutions
- Univerzitet Crne Gore