Dual-Mode Fluorescence and Colorimetric Detection of Bilirubin Using a Curcumin–Methylene Blue Metal–Organic Framework Composite

Description

Spectral and Characterization data asscociated with project entitled, Dual-Mode Fluorescence and Colorimetric Detection of Bilirubin Using a Curcumin–Methylene Blue Metal–Organic Framework Composite.

Steps to reproduce

The data were generated through synthesis, characterization, and analytical evaluation of a dual‑mode MOF‑based bilirubin sensor. Amine‑functionalized UiO‑66‑NH₂ was synthesized via a solvothermal method using ZrOCl₂·8H₂O and 2‑aminoterephthalic acid in DMF, followed by solvent exchange and activation under vacuum. Curcumin was encapsulated into the activated MOF by diffusion from ethanol, and methylene blue was introduced during sensing experiments to enable complementary colorimetric readout. Structural and physicochemical characterization data were obtained using powder X‑ray diffraction (PXRD), nitrogen adsorption (BET surface area), FT‑IR spectroscopy, thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). Optical response data were collected using UV–vis spectrophotometry and fluorescence spectroscopy under controlled pH (PBS, pH 7.4) and incubation conditions. Bilirubin calibration, selectivity studies, and limits of detection were determined from fluorescence and absorbance measurements using freshly prepared bilirubin standards. Real‑sample data were obtained by spiking bilirubin into diluted pooled human serum and quantified using external calibration and standard‑addition protocols. All measurements were performed in triplicate or quadruplicate using fixed instrumental settings to ensure reproducibility.

Institutions

• Virginia State University

  Virginia, Petersburg

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