Neuron-derived vesicular circMagi1 restrains vascular repair after ischemic stroke by promoting TRIM65-mediated ANXA2 degradation

Description

This dataset contains the multi-omics datasets and molecular validation data underlying the manuscript entitled “Neuron-derived vesicular circMagi1 restrains vascular repair after ischemic stroke by promoting TRIM65-mediated ANXA2 degradation.” The deposited data are organized into four main components: 1. Human plasma EV circRNA profiling: circRNA expression matrices used to identify circMagi1 enrichment in circulating extracellular vesicles from patients with acute ischemic stroke. 2. Single-cell RNA sequencing: transcriptomic profiles of ischemic mouse brain tissue used to characterize cell-type-specific responses to siCircMagi1-RVG-EV treatment, with particular emphasis on endothelial-state transitions. 3. RNA-protein interaction analyses: RAP-MS datasets identifying circMagi1-associated proteins and ANXA2 RIP-seq datasets characterizing ANXA2-associated RNA transcripts. 4. Structural modeling: AlphaFold 3-predicted three-dimensional models of the circMagi1-ANXA2 complex used to identify candidate RNA-protein interaction interfaces and guide subsequent mutational validation. Together, these datasets support the identification of vesicular circMagi1 as a stroke-associated RNA signal, the characterization of its effects on endothelial repair, and the mechanistic analysis of the circMagi1-ANXA2-TRIM65 pathway.

Institutions

• Institute of Biophysics

  Beijing, Beijing
• Shenzhen University of Advanced Technology

  Guangdong, Shenzhen

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