Filter trapping protocol to detect aggregated proteins in human cell lines
Published: 7 July 2022| Version 1 | DOI: 10.17632/hb8mx8xt2n.1
Contributor:
Shintaro IwasakiDescription
The loss of protein homeostasis results in cytotoxic protein aggregates, a common hallmark of aging and neurological diseases. Here, we present an adjusted filter trapping assay protocol to detect global aggregated proteins in human cell lines, via a high-sensitive protein staining method. This protocol also details an alternative approach to monitor specific protein aggregates trapped in the filter membrane, by subsequent immunoblotting of ectopically expressed and endogenous proteins. For complete details on the use and execution of this protocol, please refer to (Chhipi-Shrestha et al., 2021).
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