Flow cytometry statistical outputs and raw data

Description

Flow cytometry output of the analysis of Tmesipteris oblanceolata.

Steps to reproduce

About 1 cm2 of fresh leaf material of the study species and the calibration standard species were chopped together in 2 ml of nuclei isolation buffer34, filtered through a 100 μm Celltric filter (Sysmex), and stained by adding 100 μl of 1 mg ml−1 propidium iodide solution. Nuclei suspensions were then incubated for c. 20 min on ice prior to analysis. The relative fluorescence of stained nuclei was acquired using a CyflowSL Sysmex flow cytometer fitted with a 100 mW green (532 nm) solid-state Cobalt Samba laser.

Institutions

Categories

Funding

Licence