RESPAN Example Datasets and Results
Published: 12 February 2025| Version 2 | DOI: 10.17632/hdvsw62w5r.2
Contributors:
Luke Hammond, Description
Five example neuron datasets acquired with a Yokogawa W1 spinning disk confocal mounted on a Nikon Ti2 inverted microscope controlled with NIS-Elements. Images were acquired with a 100× 1.35 NA SR HP Plan Apo silicone immersion objective and a Prime BSI sCMOS camera (Teledyne Photometerics), resulting in pixel size of 65nm and a z-step size of 150nm. Example expected results from RESPAN processing are also included.
Files
Steps to reproduce
Analysis outputs can be reproduced by using the RESPAN (https://github.com/lahammond/RESPAN) analysis pipeline.
Institutions
Columbia University, Ohio State University
Categories
Neuroscience, Neuron, Automated Segmentation