PLI50 with qacA/B

Published: 2 March 2026| Version 1 | DOI: 10.17632/hh6xp3dmhc.1
Contributor:
Cheng-Mao Ho

Description

Schematic maps of pLI50 constructs carrying either qacA (A) or qacB (B). Inserts were cloned between EcoRI and BamHI sites under their native promoter.

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Steps to reproduce

The coding sequences of qacA and qacB, including their native promoter regions, were synthesized de novo based on GenBank accessions NC_007931.1 and NC_010419.1. To facilitate cloning, EcoRI (GAATTC) and BamHI (GGATCC) restriction sites were appended to the 5′ and 3′ termini of each construct.

Categories

Microbiology, Antibiotic Resistance

Funders

  • Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung, Taiwan.
    Grant ID: TTCRD110-30

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