Two-parameter single molecule analysis for measurement of chromatin mobility

Published: 26 December 2020| Version 1 | DOI: 10.17632/hxnhtttxpk.1
Contributor:
Jonathan Lerner

Description

This protocol provides a two-parameter analysis of live-cell Single Molecule Tracking (SMT) trajectories of Halo-tagged histone and unveils a chromatin mobility landscape composed of five chromatin types ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not. For complete details on the use and execution of this protocol, please refer to Lerner et al., 2020.

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Institutions

  • University of Pennsylvania

Categories

Molecular Biology, Microscopy, Single Molecule Imaging, Single Molecule Method

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