Inhibition of SIRT7 Overcomes Radioresistance in Pancreatic Neuroendocrine Tumors by Reactivating MEN1 Expression
Published: 6 March 2026| Version 1 | DOI: 10.17632/j2rjmxyyfw.1
Contributor:
Jianyun JiangDescription
Enrichment of DNMT1 at the MEN1 promoter was calculated relative to input DNA and normalized against IgG isotype control immunoprecipitations. To examine region-specific recruitment, we performed ChIP-qPCR targeting two distinct regions within the MEN1 promoter (P1 and P2), together with a negative control region (P3). The primer sequences used for qPCR are listed below: P1 region: Forward 5′-GCCAATCCCTGAGTATCTC-3′; Reverse 5′-CGCTGAGCCTGGAAATAG-3′ P2 region: Forward 5′-ATCACAGCTACTCGGGAG-3′; Reverse 5′-ACACGGTCTTTCTCTTTCG-3′
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Institutions
- Fudan University Shanghai Cancer CenterShanghai, Shanghai
Categories
Chromatin Immunoprecipitation