Genotype images for Yuri et al iScience

Published: 4 November 2024| Version 1 | DOI: 10.17632/j56pkkwh97.1
Contributor:
Shunsuke YURI

Description

The genotype results for established ESCs and mouse.

Files

Steps to reproduce

For detecting the Mesp1-KO and Mesp1/2-DKO genotypes, DNA fragments were amplified using GoTaq (Promega) for 40 cycles to detect null or WT alleles under the following conditions: 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 60 s.

Institutions

Kokuritsu Choju Iryo Kenkyu Center Kenkyujo

Categories

Genotyping

Funding

Japan Society for the Promotion of Science

Grant Numbers 23K18577 and 24K01948 to A.I. and 18K06031 and 22K06067 to S.Y.

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