AutoSpectral: OMIP-102 unmixing
Published: 27 October 2025| Version 1 | DOI: 10.17632/j6yvfgdrcp.1
Contributors:
Oliver Burton, , Description
Re-unmixing of OMIP-102 using AutoSpectral. Examples are provided using per-cell autofluorescence extraction (scAF-tagged files), or, additionally, with per-cell fluorophore optimization after single-cell autofluorescence extraction. The latter is provided with the exact computation approach ("slow") or the "fast" approximation. The intermediate Woodbury rank-one updating approach is not provided due to limits on the storage space.
Files
Steps to reproduce
See GitHub links and OMIP-102: https://pubmed.ncbi.nlm.nih.gov/38634730/
Institutions
University of Cambridge
Categories
Flow Cytometry, Spectral Unmixing
Funding
KU Leuven
C1 Program
European Research Council
No 874707 (EXIMIOUS)
Wellcome Trust
222442/A/21/Z
UK Research and Innovation
MR/Y004450/1 (IMMPROVE)