Supplementary figures and table for: Autologous split-thickness skin graft–acellular dermal matrix composite transplantation promotes physiologic skin remodeling: A paired, controlled study in rats
Description
This dataset contains the 11 supplementary figures and 1 supplementary table accompanying the above-named article. The study tested the hypothesis that compositing an ultrathin (0.2-mm) autologous split-thickness skin graft (STSG) with human acellular dermal matrix (ADM) restores a dermal scaffold beneath the graft and thereby reduces wound contraction and promotes physiologic, rather than fibrotic, skin remodeling. Data were generated in a within-animal paired model: each of 20 male Sprague-Dawley rats received two dorsal full-thickness skin defects randomized to STSG alone or STSG+ADM, and wounds were followed on days 3, 7, 14, 21, and 28. The files comprise representative source images underlying the quantitative analyses reported in the article: Supplementary figures 1-2 document the surgical model and the modified thickness-calibrated dermatome; Supplementary figure 3 shows hematoxylin-eosin histology of normal skin and harvested grafts; Supplementary figure 4 shows the gross appearance of grafted wounds over time; Supplementary figure 5 shows body weight curves (mean ± SD) as a welfare/toxicity control; Supplementary figures 6-9 show, respectively, hematoxylin-eosin histology, collagen remodeling (Masson trichrome and Picrosirius red under polarized light, in which yellow-orange birefringence indicates type I and green indicates type III collagen), CD34 immunostaining of angiogenesis, and cytokeratin 14 immunofluorescence of re-epithelialization; Supplementary figure 10 shows scanning electron microscopy of the ADM architecture; and Supplementary figure 11 shows rat epidermal keratinocytes cultured on ADM, including CCK-8 proliferation data. Supplementary table 1 lists the primer sequences used for reverse transcription–quantitative PCR. These data support the article's main findings: compared with STSG alone, the composite graft reduced wound contraction by 19-43 percentage points, preserved graft thickness, and maintained total collagen deposition statistically indistinguishable from unwounded skin during peak remodeling. The images are provided for transparency and reviewer reference; quantitative endpoints derived from them (wound area, contraction rate, graft thickness, collagen area and type I/III ratio, CD34+ vessel density, CK14+ epithelial area, and gene expression) are reported in the article text, figures, and tables. Researchers may reuse these images as reference material for comparable grafting and dermal-substitution studies; Picrosirius red images were uniformly color-adjusted as disclosed in the article's figure legends.