Streptococcus suis serotype 2 evades autophagic clearance via mitochondrial ROS-induced LMP and ATP6V1B1 downregulation
Published: 11 July 2026| Version 3 | DOI: 10.17632/j99yg4nttw.3
Contributors:
Fei Liu, Shuzhi Zhang, Pei Wu, Yuyu Zhang, Jianda Li, Luogang Ding, Yulin Xu, Zhi Chen, Wenbo Sun, Jiang Yu, Jiaqiang WuDescription
Original WB image, ARRIVE GUIDLINE-author checklist
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Total protein lysates were prepared with RIPA buffer containing protease inhibitors, quantified by BCA eagents (Thermo Scientific, A55860), separated by SDS-PAGE, and transferred to PVDF membranes. PageRuler™ Prestained Protein Ladder (Thermo Scientifi; 26616) was used as the molecular weight standard. After blocking with 5% skim milk, membranes were incubated with primary antibodies overnight at 4°C, followed by HRP-conjugated secondary antibodies for 1 h at room temperature. Protein bands were visualized using ECL (Thermo Scientific, 32209) and quantified with ImageJ software.
Institutions
- Shandong Academy of Agricultural SciencesShandong, Jinan
Categories
Bacteria, Pathogenesis