Chlorantraniliprole Impairs Mobility of Males in Bombus terrestris
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The data of Chlorantraniliprole Impairs Mobility of Males in Bombus terrestris
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Insects Commercial colonies of B. terrestris were obtained from Hengshui Woofun Biotechnology Co., Ltd. (Hebei, China) and maintained at 26 ± 1°C, 60 ± 5% RH, 0:24 L:D. Newly emerged males were randomly distributed into rearing chambers (20×20×20 cm) with maximum 40 individuals per chamber. Pesticide Exposure Analytical-grade CAP (97.7% purity; Sinochem Group) was dissolved in DMSO to prepare 10 mg/g stock solutions, then diluted in 50% w/v sucrose solution to achieve 1.0 μg/g CAP with 0.01% (v/v) DMSO in all groups. Following OECD Guidelines 245 and 246, test solutions were provided ad libitum with daily replacement during the 10-day exposure period (day 2-11 post-emergence), after which bees received untreated sucrose solution. The Effects of CAP on the Crawling Ability of Males A locomotor assessment apparatus featured a single-bee crawling tunnel (70 × 1 × 1 cm) with photoelectric sensors at 50 cm intervals and a light gradient system (5,000-150,000 LUX). At 26.5 ± 1°C, males underwent 30-minute light adaptation before testing. Males were evaluated for tunnel traversal over 3 minutes (100% of controls and 98.8% of treated group completed), with locomotor speed calculated from sensor data. Data included baseline and daily measurements throughout exposure (n=50 per group). The Effects of CAP on the Flight Ability of Males Flight behavior was evaluated in control and CAP-exposed (1.0 μg/mL) bumblebees at 26.5±1℃ with light intensity >5,000 LUX. Individuals completing <200 rotations were excluded as outliers. The Effects of CAP on the Flight Muscle Tissue Males post-mating trials were cryopreserved in liquid nitrogen and thoracic segments dissected. Cryosections (10 μm) were obtained using a Leica CM1950 cryostat (chamber: -21℃; specimen head: -16℃). Morphological analysis used a Nikon 80i microscope at 4× and 40× magnification for nuclear density measurements.
Institutions
- Xinjiang University
- Chinese Academy of Agricultural Sciences
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Funders
- Innovation Project of Xinjiang Academy of Agricultural Reclamation ScienceGrant ID: NCG202507