Ngoc Linh ginseng fermentation

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Raw data of Ngoc Linh ginseng fermentation project

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1 - Ginsenoside profiles by HPLC-MS/MS The ginsenoside profile was quantified using a Shimadzu LC-40D liquid chromatography system (Shimadzu, Kyoto, Japan) coupled with a phenyl column (250 mm  4.6 mm; 5.0 µm), an SPD-20A UV detector and an LC-40D pump. The powder was extracted with methanol, and sonicated at 45 °C for 15 min before being filtered through a 0.45-µm PTFE syringe. The mobile phase consisted of water (solvent A) and acetonitrile (solvent B) with the following elution gradient: 80% A for 5 min, 77% A for 5–20 min, 70% A for 20–25 min, 60% A for 25–32 min, 50% A for 32–38 min, 15% A for 38–52 min, and 80% A for 52–61 min. The flow rate was fixed at 1 mL/min and the column temperature was maintained at 35 °C. The UV wavelength used for detection of the compound was 203 nm and the injection volume was 10 µL. The ginsenoside standards included Rg1, Re, Rf, Rb1, Rc, Rb2, Rd, Rg3, and Mr2. 2 - Organic acid composition The organic acid composition of the samples was analyzed using an Agilent 1260 Infinity HPLC system (Milford, MA, USA) coupled with a C18 column (100 Å, 4.6 × 250 mm, 5 μm) and UV detector. The samples were diluted and treated with potassium hexacyanoferrate (10.6%) and zinc sulfate (30%) before filtration through a 0.45 µm membrane. The mobile phase used was 0.010 M sodium dihydrogen phosphate pH 2.80 solution which was passed through the column for 17 min. The flow rate was fixed at 0.8 mL/min and the column temperature was maintained at 25 °C. The UV wavelength used for detection of the compound was 210 nm and the injection volume was 5.0 µL. 3 - Amino acid composition The amino acid composition of the sample was analyzed using the L-8900 automatic AAA system (Hitachi, Tokyo, Japan) connected with an HPLC column with ion-exchange resin 2622 PF (60 × 4.6 mm, 3 μm). Sample preparation was according to the manufacturer's protocols and included protein hydrolysis, pH adjustment, and filtration. In addition, cystine and methionine were differentiated from the other 17 amino acids in terms of sample preparation. Specifically, these two amino acids were oxidized with performic acid before being hydrolyzed with HCl for 24 h, adjusted to pH 2.2 with citrate buffer, and filtered through a 0.45 µm membrane. The filtered sample (10 µL) was injected into the system at a column temperature of 57 °C and analyzed using five mobile phase with ninhydrin reagent according to the manufacturer's instructions.

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