Journal of Comparative Pathology

Data of the histopathological findings of a cohort of 35 lowland tapirs

Supplement 1. Primary stereological data on volume fraction of connective tissue and cross-sectional area of classical lobules in all investigated samples. The following abbreviations are used: VV(ICT) – volume fraction of interlobular connective tissue (connective tissue surrounding liver lobule), VV(PSCT) – volume fraction of perisinusoidal connective tissue (connective tissue located at the lobular sinusoids), VV(PCCT) – volume fraction of pericentral connective tissue (connective tissue surrounding central vein), VV(CT) – volume fraction of total connective tissue (sum of all connective tissue volume fractions). Supplement 2. Calculating sample sizes needed to detect a certain expected relative increase in the connective tissue fraction in various hepatic lobes. VV(CT) – volume fraction of connective tissue (total connective tissue irrespective of its localization), n – number of tissue blocks in each of the groups that are to be compared. Sample sizes were calculated according to Chow et al. (power=0.8; significance level=0.05) (2008). The data are presented for both sexes. Supplement 3. Calculating sample size needed to detect a certain expected relative increase in the connective tissue fraction in various regions of interests (ROIs) within hepatic lobes. Peripheral ROI – region at the periphery of a liver lobe, paraportal ROI – region of a lobe near the porta hepatis, paracaval ROI – region of a lobe adjacent to the caudal vena cava, VV(CT) – volume fraction of connective tissue (total connective tissue irrespective of its localization), n – number of tissue blocks in each of the compared groups. Sample size were calculated according to Chow et al. (power=0.8; significance level=0.05) (2008). The data are presented for both sexes and each ROI within each hepatic lobe.