Raw data - Ovariectomy induces regression of hyperplastic uterus in female dogs

Description

This dataset contains raw and processed data from the original research study entitled “Ovariectomy induces regression of hyperplastic uterus in female dogs”, which evaluated the uterine involution in bitches diagnosed with cystic endometrial hyperplasia (CEH) after bilateral ovariectomy. The hypothesis tested was that the removal of steroidal hormonal influence through ovariectomy would induce regression of endometrial and glandular hyperplasia, observable macroscopically, histologically and immunohistochemically. Data were collected over a 6-month period from 20 nulliparous bitches in diestrus or anestrus, selected and monitored by the Veterinary Hospital of the Federal University of Viçosa. The study included macroscopic analysis of the uterus, ultrasonographic measurements, histomorphometric evaluation, and immunohistochemistry for estrogen (ER) and progesterone receptors (PR), as well as the proliferation marker Ki-67. Each dog underwent an initial ovariectomy with right hysterectomy, and after six months, a left hysterectomy for comparative analysis. Uterine tissues were collected at both time points. The dataset is composed of multiple files in .xlsx format, organized by subject ID and categorized according to: ultrasonographic uterine measurements before and after surgery, morphometric histological data (including uterine wall thickness, endometrial and myometrial measurements, glandular diameter and epithelial heights), immunohistochemistry scores (ER, PR, Ki-67), and CEH grading. Tables summarizing averages and standard deviations across subjects, as well as raw data tables, are included. Each variable is labeled, and a dictionary of terms is provided to ensure interpretability. The findings derived from these data revealed that 88% of bitches showed at least a 50% reduction in total uterine wall thickness, with corresponding reductions in the endometrial and myometrial layers. Significant decreases in the expression of ER and PR receptors, as well as Ki-67, were observed in most subjects after six months. These findings support the conclusion that ovariectomy induces substantial uterine regression and can be considered an effective approach for CEH management in bitches. This dataset may be valuable for researchers investigating uterine physiology, veterinary reproductive surgery, hormonal receptor dynamics, or canine endometrial pathology.

Steps to reproduce

The data were generated following a controlled experimental protocol approved by the Animal Ethics Committee of the Federal University of Viçosa (protocol 31/2022). Twenty healthy nulliparous bitches in diestrus or anestrus were selected based on physical examination, hematological and biochemical analysis, vaginal cytology, and abdominal ultrasonography. Ovariectomy and uterine horn biopsies were performed under general anesthesia using standardized surgical techniques. Uterine samples were collected during two surgeries: the first involving ovariectomy and right hysterectomy, and the second—performed six months later—consisting of left hysterectomy through the same surgical access. Histological sections (5 µm) were stained with hematoxylin and eosin using routine histopathological procedures. Immunohistochemistry was conducted using antibodies against estrogen receptor (clone PPG5/10), progesterone receptor (polyclonal, Dako), and Ki-67 (clone MIB-1, Dako). Slides were processed using standardized antigen retrieval and blocking protocols and analyzed under an Opticam 0500R light microscope. Quantification of Ki-67 expression was performed by manual counting of positively stained cells across three regions per slide. Estrogen and progesterone receptor expression were semi-quantitatively scored. Histomorphometric measurements—including uterine wall thickness, glandular diameter, epithelial height, and endometrial thickness—were performed using ImageJ software. Data were analyzed statistically using SAS OnDemand software with paired t-tests, Wilcoxon tests, and correlation analysis, as appropriate.

Institutions

• Universidade Estadual Paulista Julio de Mesquita Filho - Campus de Jaboticabal

  SP, Jaboticabal
• Universidade Federal de Vicosa

  MG, Vicosa
• Universidade Federal de Goias

  GO, Goiania
• Universidade Federal do Para

  PA, Belem

Categories

Funders

• National Council for Scientific and Technological Development

  Federal District, Brazil
• Fundação de Amparo à Pesquisa do Estado de Minas Gerais

  Minas Gerais, Brazil
• Coordenação de Aperfeicoamento de Pessoal de Nível Superior

  Federal District, Brazil

Licence