Data for: Dynamic integration of morphogen and juxtacrine signalling specifies cell fate at single-cell resolution
Description
This dataset contains the full set of confocal microscopy images associated with the manuscript and the raw numerical data underlying all experimental quantifications and statistical analyses. Computational modelling code, simulation outputs, and associated analyses are deposited separately at https://doi.org/10.5281/zenodo.20306264
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Steps to reproduce
Drosophila melanogaster strains were reared on standard cornmeal medium and analysed during early pupal development as described in the associated manuscript. Eye-optic lobe complexes were dissected, fixed, stained (immunohistochemistry or in situ hybridisation chain reaction), and imaged using Zeiss 800 and 880 confocal microscopes. Live-imaging experiments were performed using an Olympus FV1000MPE multi-photon microscope. Raw numerical data were extracted from confocal image datasets using Fiji-ImageJ. Quantifications included fluorescence intensity measurements, cell counts and neuronal subtype quantifications. Statistical analyses were performed using GraphPad Prism 9. All quantifications were performed blinded to experimental condition. This repository contains the full set of confocal microscopy images associated with the manuscript together with the raw numerical values underlying all experimental quantifications and statistical analyses. Detailed experimental procedures, fly genotypes, imaging conditions, quantification methods and statistical analyses are provided in the STAR Methods section, Table S1, and associated supplementary materials of the manuscript. Computational modelling code, simulation outputs, and associated analyses are deposited separately at https://doi.org/10.5281/zenodo.20306264.
Institutions
- University College LondonEngland, London