Gilbués FASTQ files

Published: 15 November 2021| Version 1 | DOI: 10.17632/k335pxb8h4.1
Contributor:
Arthur Prudêncio de Araujo Pereira

Description

Raw FASTQ data of the paper entitled: Land degradation affects the composition, structure, and diversity of the microbial community in the Brazilian semiarid

Files

Steps to reproduce

Total soil DNA was extracted from 0.5 g of soil using the DNeasy PowerLyzer PowerSoil Kit (Qiagen, CA, USA), according to the manufacturer's instructions. The quality of the DNA was checked through of spectrophotometry (Nanodrop ND-1000) and electrophoresis (0.8% agarose gel). The V4 region of the 16S rRNA gene was amplified using the Platinum Taq polymerase High Fidelity (Invitrogen, Carlsbad, CA, USA) and primer set 515F (5'-GTGCCAGCMGCCGCGGTAA-3’) and 806R (5'-GGACTACHVHHHTWTCTAAT-3’) (Caporaso et al., 2011), according to the following program: 95°C for 4 min, 60°C for 1 min, 72°C for 2 min, followed by 25 cycles at 94°C for 1 min, 60°C for 1 min, and 72°C for 2 min. PCR concentration was measured by Qubit™ fluorometer (Invitrogen 2.0), purified using calibrated Agencourt AMPure XP beads (Beckman Coulter, Brea, CA, USA) and paired-end sequenced using an Illumina MiSeq Reagent Kit v2 (300-cycles, 2 × 150 bp) into an Illumina MiSeq sequencer (Illumina, 2013) at the Genomic and Bioinformatic Facility Centre (CeGenBio) of the Federal University of Ceara, Brazil.

Institutions

Universidade Federal do Ceara

Categories

Illumina MiSeq

License