Data for: MDA5 and LGP2 acts as a key regulator though activating NF-κB and IRF3 in RLRs signaling of Mandarinfish
Characterization of the effects of MDA5 and LGP2 on induction of IFN-β, NF-𝜅B and IRF3. MDA5 293T cells and LGP2 293T cells were seeded in 6 well plates and treated with 10ng/ml tetracycline, respectively, after seventy-two hours culturing, the cells were stimulated with poly I:C (1mg/mL) or LPS (1mg/mL). Ten hours later, the cells were treated with RIPA to carry out the western blot analysis. Gray value detection of western blot were repeated at least three times, and rubber map of western blot was used.