Data of RNA sequencing and hydroxyproline level from an experiment examining the influence of Poria cocos polysaccharides on NASH mice
Description
The differentially expressed genes shared by MCS and HP groups compared to MCD group (Table S1), KEGG pathways enriched by HP group compared to MCD group (Table S2) and the hydroxyproline level in all mice (Fig. S1).
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Steps to reproduce
C57BL/6 mice received 4 weeks of methionine and choline deficient (MCD) diet to establish nonalcoholic steatohepatitis model or methionine-choline-sufficient (MCS) diet as controls. Mice were assigned to the MCS group, MCD group, low-dose Poria cocos polysaccharides (PCP) (LP) group and high-dose PCP (HP) group, and were administered corresponding medications via gavage. The hepatic hydroxyproline and RNA sequencing (RNA-Seq) was performed. The thresholds for differential expression shared by MCS and HP groups compared to MCD group analysis were established at |log2 fold change|> 1 and P value < 0.05.The hypergeometric test was used to calculate the KEGG pathway enrichment analyses, which was conducted using the KEGG pathway database (http://www.kegg.jp/). The hydroxyproline level in all mice was detected by the assay kit (A030-2-1, Nanjing Jiancheng Bioengineering Institute, China).
Institutions
- Shanghai University of Traditional Chinese Medicine