Prophylactic Effects of Chlorogenic Acid Against Cyclophosphamide-Induced Kidney and Bladder Damage in Rats
Description
This dataset contains the raw data underlying the study "Prophylactic Effects of Chlorogenic Acid Against Cyclophosphamide-Induced Kidney and Bladder Damage in Rats". Eighteen male Sprague-Dawley rats (250–300 g) were allocated to three groups (n = 6 per group): a control group (CG), a cyclophosphamide group (CPG; single intraperitoneal dose of 150 mg/kg on day 7), and a cyclophosphamide plus chlorogenic acid group (CP+CGAG; 100 mg/kg/day chlorogenic acid orally on days 1–7 followed by the same cyclophosphamide dose on day 7). The file contains individual animal values for body weight (days 1 and 8), right kidney and bladder weights, kidney histopathological scores (tubular degeneration, glomerular degeneration, oedema-haemorrhage), bladder damage score, apoptotic indices of both tissues determined by TUNEL, plasma blood urea nitrogen and creatinine, and oxidative stress and inflammatory markers (MDA, TOS, TAS, OSI, SOD, CAT, GSH, TNF-α, MPO) measured in kidney and bladder homogenates. Group definitions, scoring criteria, units and ethical approval details are given in the README sheet of the file
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Eighteen male Sprague-Dawley rats (250–300 g) were randomly allocated to three groups (n = 6 per group). The control group received 2 mL phosphate-buffered saline by oral gavage on days 1–7 and a single intraperitoneal injection of 2 mL saline on day 7. The cyclophosphamide group received a single intraperitoneal injection of 150 mg/kg cyclophosphamide on day 7. The cyclophosphamide plus chlorogenic acid group received 100 mg/kg/day chlorogenic acid orally on days 1–7 followed by the same cyclophosphamide dose on day 7. Animals were weighed on days 1 and 8 and sacrificed under ketamine anaesthesia. Blood was collected into EDTA tubes; the right kidney and the bladder were removed and weighed. Half of each tissue was fixed in 10% formalin for histopathological evaluation (haematoxylin-eosin, periodic acid-Schiff and TUNEL staining) and the remaining half was stored at −80 °C for biochemical analyses. Tissue homogenates were prepared in phosphate-buffered saline and centrifuged at 1,800 × g for 10 min. Total protein was measured by the bicinchoninic acid method. MDA was determined by the thiobarbituric acid method; SOD, CAT, GSH, TNF-α and MPO were measured by ELISA; TOS and TAS were measured using commercial colorimetric kits, and OSI was calculated from these values. Plasma BUN and creatinine were measured on an automated biochemistry analyser. Full details of scoring criteria and units are given in the README sheet of the dataset file.
Institutions
- Recep Tayyip Erdoğan UniversityRize Province, Rize