Identification of phosphorylated peptides in Maf1 from Leishmania major cells in exponential growth and nutritional stress
Description
The goal of this experiment was to identify phosphorylated residues in LmMaf1 in mid-logarithmic phase and stationary phase promastigotes from L. major. This data set contains the mass spectrometry results obtained from proteins obtained by Tandem Affinity Purifications using a L. major cell line that expresses the recombinant Maf1-PTP protein. Obtained peptides were digested with trypsin and chemotrypsin. All the *.csv files contain the peptide sequences and the proteins identified. For these experiments, we used a Synapt G2-Si mass spectrometer (Waters) in High Definition MS/MS mode and compared using ProteinLynx Global SERVER v3.0.3 (Waters, Milford, MA) against a L. major Friedlin strain *.fasta database (downloaded from UniProt, 8038 protein sequences, last modified 27th June 2018).
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Institutions
- Universidad Nacional Autonoma de Mexico Facultad de Estudios Superiores Iztacala