Lactobacillus paracasei Expressing Porcine Trefoil Factor 3 and Epidermal Growth Factor: A Novel Approach for Superior Mucosal Repair
Description
Inflammatory bowel disease significantly impairs patients’ physical health and quality of life, presenting a major challenge in modern healthcare. This chronic condition, characterized by persistent gastrointestinal inflammation, not only affects physiological functions but also imposes considerable psychological and socioeconomic burdens on affected individuals and healthcare systems. The primary objective of this study was to investigate the therapeutic potential of orally administered porcine trefoil factor 3 and epidermal growth factor delivered by Lactobacillus paracasei 27-2 for intestinal mucosal repair. Our experimental results demonstrated that this novel delivery system signifi-cantly enhanced the proliferation and migration capabilities of Immortalized Porcine Enterocyte Cell line J2, suggesting its promising application in promoting intestinal mucosal regeneration and repair. Moreover, the oral administration of the recombinant L. paracasei 27-2 strains could effectively alleviate the symptoms of colitis induced by dextran sulfate sodium in mice and significantly improve the integrity of the intestinal mucosa. In conclusion, using Lactobacillus paracasei 27-2 as an oral delivery vector to ex-press trefoil factor and epidermal growth factor shows great potential as a novel strat-egy for treating inflammatory bowel disease.