Supplemental figures and tables for EGFP-SD and PD-1-SD degradation study

Description

Differential protein degradation with a super-degron tag and thalidomide analogs: EGFP is degraded in wild-type mouse cell, while PD-1 requires CRBN humanization, providing new insights into T cell regulation. The dataset includes all supplementary figures and tables.

Steps to reproduce

Detailed experimental procedures are described in the STAR Methods section of the associated manuscript. Briefly, super degron-tag fused EGFP-expressing cells were generated. Furthermore, super degron-tag knock-in mice were generated using CRISPR-Cas9, and EGFP or PD-1 degradation was assessed by flow cytometry after iberdomide treatment.

Institutions

• Kyoto Daigaku

Categories

Funders

• Kyoto University

  Japan

  Grant ID: 2025Y
• Japan Society for the Promotion of Science

  Japan

  Grant ID: JP21K19188
• Japan Society for the Promotion of Science

  Japan

  Grant ID: JP22H04922
• Japan Society for the Promotion of Science

  Japan

  Grant ID: JP20H03171

Licence