Response of DPR in Farmland Soils to Warming
Description
Responses of N₂O emission potential driven by denitrification in different types of farmland soils to warming.
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To capture a broad climatic gradient, 18 soil samples (0–20 cm depth) were gathered from agricultural fields across six eastern Chinese provinces, spanning a latitudinal gradient from 19°53′N (Hainan) to 45°31′N (Heilongjiang) (Figure S1). The geographical range encompasses temperate, subtropical, and tropical monsoon climate zones, representing diverse agricultural ecosystems. At each province, three representative sites were selected. Post-collection, visible residues were cleared and samples passed through 2 mm sieves. Depending on the analysis type, soil was stored at 4°C (slurry assays), -20°C (DNA work), or air-dried (chemical analysis). Detailed site and physiochemical information are provided in Table S1 and Table S2. Soil texture was analyzed using the hydrometer method (Gee and Bauder, 1986). Soil Inorganic nitrogen (NO3−-N and NH₄⁺-N) concentrations were quantified via continuous flow analysis. Soil pH was measured in a 1: 2.5 (soil: water) suspension using a pH meter (PHS-3C mv/pH detector, Shanghai, China). Dissolved organic carbon (DOC) and dissolved organic nitrogen (DON) were determined using a UV-persulfate TOC analyzer (Phoenix 8000,Teledyne-Tekmar, USA). Available phosphorus was assessed using inductively coupled plasma optical emission spectrometry (ICP-OES, Thermo Fisher, USA) (Malmir et al., 2019). Total carbon (TC) and nitrogen (TN) contents were measured using an element analyzer (Elementar, Germany) after sample ball milling. The denitrification potential rate (DPR) was estimated via the acetylene inhibition technique (Petersen et al., 2012).
Institutions
- Institute of Applied Ecology Chinese Academy of Sciences
- China Agricultural University
- Zhejiang University