16S rDNA
Published: 29 June 2022| Version 1 | DOI: 10.17632/mjpr4v7dyd.1
Contributor:
菲妮 周Description
The present study was curcumin treatment of DSS-induced acute colitis mice and the study was divided into three groups (n = 8/group), control group (Con), model group, and treatment group. The data used mouse feces as a sample to detect the intestinal microbiota.
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Steps to reproduce
1.Paired-end reads was assigned to samples based on their unique barcode and truncated by cutting off the primer sequence； Softwares:cutadapt Version:cutadapt-1.9 2.Paired-end reads into long amplicon fragments 16S(V3+V4): Softwares:FLASH Version:FLASH-1.2.8 ITS2: Softwares:PEAR Version:PEAR-0.9.6 3. denoise Softwares:qiime2 DADA2 Version:2019.7 4. sequence alignment Softwares:qiime2 feature-classifier Version:2019.7 5. Database for taxonomy SILVA（release 132） NT-16S（2019.04.05）
Categories
rDNA Analysis, Illumina MiSeq