Quantification of the genetically modified organism "MON810 maize event" in food and feed commodities sold in the market of Kuwait using droplet digital PCR

Description

The primary goal of conducting this study is to establish a reliable analytical system in our laboratory for the routine assessment and quantification of GMOs in food and feed commodities imported to Kuwait’s market, thus contributing significantly to future food safety legislation in Kuwait. The study reports for the first time the employment of duplex droplet dPCR (ddPCR) assay to quantify the authorized maize MON810 event in two maize-derived food and feed commodities sold in the local market of Kuwait. The employed duplex ddPCR assay for the quantification of MON810 maize event was in-house verified and met the minimum performance requirements of analytical methods for GMO testing set by the European Network of GMO Laboratories (ENGL).

Steps to reproduce

1. Certified reference materials can be purchased from IRMM (Geel, Belguim). Maize-derived food and feed products can be obtained from the local market. genomic DNA extraction can be performed using a commercial GMO extraction kit (e.g. from ThermoFisher Scientifics). Quality control of extracted gDNA can be evaluated by NanoDrop Spectrophotometer, Qubit Fluorometer, and agarose gel electrophoresis. Promers and probes can be purchased from Eurofins MWG Operon (Ebersberg, Germany). Qualitative PCR can be conducted using any thermal cycler. Quantitative PCR can be conducted using the BioRad Q100/Q200 platform with integrated QuantaSoft analysis software.

Institutions

• Kuwait University

  Al Asimah, Kuwait City

Categories

Funders

• Kuwait University

  Kuwait City

Licence