Redox controls of Arf small GTPase

Description

Data sources for kinetic EPR analyses and additional data for ESI-MS and cell scratch assays are placed.

Steps to reproduce

All methodologies for the redox kinetic EPR studies were conducted under the strict anaerobic conditions. When necessary, however, atmospheric O₂ was added to the system to produce aerobic experimental conditions. Kinetic data were produced by using mant fluorescent-tagged GDP. This approach is well known for the kinetic analysis of small GTPases. The EPR analysis employed rapid freeze-quench methods, which are also well known.

Institutions

• University of Texas at Arlington

  TX, Arlington

Categories

Funders

• National Institutes of Health

  Maryland, United States

  Grant ID: R15GM152973

Licence