Acute depletion of the ARID1A subunit of SWI/SNF complexes
Steps to reproduce
Western blot of whole cell lysates for ARID1A depleted cells in a time course. Three biological replicates each time point. 30ug of lysate loaded. Rabbit polyclonal anti-ARID1A, Sigma-Aldrich HPA005456, RRID:AB_1078205, Mouse monoclonal anti-Actin, clone AC15 Sigma-Aldrich A5441, RRID:AB_476744. Licor image with secondary IRDye 800CW goat anti rabbit, LICOR 926-32211 and Alexa Fluor 680 goat anti mouse, Invitrogen A21058.