ARID1A depletion

Published: 25 October 2021| Version 2 | DOI: 10.17632/mvnz46kfwn.2
Contributors:
Nicola Wiechens,
Tom Owen-Hughes

Description

Acute depletion of the ARID1A subunit of SWI/SNF complexes

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Steps to reproduce

Western blot of whole cell lysates for ARID1A depleted cells in a time course. Three biological replicates each time point. 30ug of lysate loaded. Rabbit polyclonal anti-ARID1A, Sigma-Aldrich HPA005456, RRID:AB_1078205, Mouse monoclonal anti-Actin, clone AC15 Sigma-Aldrich A5441, RRID:AB_476744. Licor image with secondary IRDye 800CW goat anti rabbit, LICOR 926-32211 and Alexa Fluor 680 goat anti mouse, Invitrogen A21058.

Categories

Proteomics, Genome Sequencing

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