Flavonoids profile in methanolic extracts from ragweed-pollen samples by LC-MS/MS

Published: 24 May 2022| Version 1 | DOI: 10.17632/n4hscrf6nd.1
Contributor:
XIAOJIE CHENG

Description

Flavonoids profile in methanolic extracts from ragweed-pollen samples has been characterized by LC-MS/MS. A total of 62 flavonoid metabolites were identified and listed.

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Steps to reproduce

1. Methanolic extracts from ragweed-pollen samples were prepared. 2. Samples were then analyzed in triplicate by Ultra Performance Liquid Chromatography (UPLC) Ultra-High Resolution (UHR) tandem quadrupole/Time-of-Flight (QqToF) mass spectrometry (MS). 3. From the exported MS data, flavonoids were identified using pure standards of kaempferol-7-O-glucoside, kaempferol, and quercetin (Extrasynthese, France), by library MS/MS match as described in Bertić et al. (2021).

Institutions

Helmholtz Zentrum Munchen Institut fur Biochemische Pflanzenpathologie

Categories

Natural Sciences

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