Sero-epidemiological study of the rotavirus VP8* protein from different P[8] genotype lineages in Valencia, Spain [Dataset]

Description

Supporting data to figure 4. Serum dilution screening. The figure shows the mean absorbance curves versus serum dilution for A) lineage I, B) lineage II, C) lineage III and D) lineage IV. Non-linear regression curves were fitted using a four-parameter logistic (4PL) model. Data points represent individual replicates, while diamonds and error bars represent mean ± SEM. The horizontal dashed line at Y = 0.2 indicates the lower threshold of acceptable absorbance. Supporting data to figure 5. Recognition pattern distribution of the VP8 protein from lineages I to IV of the P[8] genotype. Vertical bars indicate the number of samples exhibiting each recognition pattern, represented by the connected dots below, which show the combinations of recognized lineages. Horizontal bars on the left represent the total number of reactive samples for each individual lineage. Supporting data to figure 6. Distribution of serum reactivity to P[8] lineages by age group. Each violin plot represents the dispersion and median of the recognition percentage for each age group within each lineage. Statistically significant differences between age groups for each lineage are indicated as follows: * p < 0.05, ** p < 0.01, *** p < 0.001. Significance was determined using Dunn’s test with Bonferroni correction, following the Kruskal-Wallis analysis. Supporting data to figure 7. Heatmap of cross-reactivity. The Y-axis represents sera from each of the four groups of immunized mice: P[8]LI (8.1.1–8.1.6), P[8]LII (8.2.1–8.2.6), P[8]LIII (8.3.1–8.3.6), and P[8]LIV (8.4.1–8.4.6). The X-axis represents the proteins from different lineages used in the ELISA assays.

Institutions

• Universitat de València

  Valencia, Valencia

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