Seasonal variation in the positional distribution of fatty acids in bovine milk fat (Supplementary Tables)
In this study, the variation in the regiospecific distribution of fatty acids (FA) of bovine milk fat throughout a year has been studied. The milk fat samples were collected on a weekly basis from May 2017 till April 2018 in the Netherlands as representative of the average bovine milk fat composition. The weekly samples were combined into months and the monthly FA compositions at the sn-2 and sn-1(3) positions were determined via enzymatic transesterification with Candida antarctica lipase B. The regiospecific distribution of FA were assessed by using intra- and interpositional approaches. Based on the intrapositional approach, majority of the sn-2 and sn-1(3) FA showed significant seasonal variation. In summer, the concentrations of blood derived FA (i.e. C18:0, C18:1 cis-9) increased and novo synthesized FA decreased. Highest concentrations of C16:0 was observed in sn-2 position of milk fat in winter whereas in summer unsaturated FA were highest at this position. Interestingly, according to interpositional distribution the esterification preferences of FA either sn-2 or sn-1(3) positions did not change significantly upon seasonal variation. The supplementary files reported in this depository are part of the manuscript by Yener et al., 2021 in Journal of Dairy Science entitled "Seasonal variation in the positional distribution of fatty acids in bovine milk fat". and can be described as below: -Supplementary Table 1 (Comparisons of the fatty acid (FA) concentrations (mol %) at the sn-2 and sn-1(3) positions in the triacylglycerol structures of bovine milk fat between seasons) -Supplementary Table 2 (Statistical analysis on the stereolocation preferences between the interpositional distribution (%) and the hypothetical proportions at the sn-2 (33.3%) and sn-1(3) (66.7%) positions in the triacylglycerol (TAG) structures) -Supplementary Table 3 (Fatty acid composition (mol %) and fat content of bovine milk fat)
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The complete description of the methods used for the assessment of the seasonal variation in the regiospecific distribution of fatty acids of bovine milk fat are explained and described in the manuscript by Yener et al., 2021 in Journal of Dairy Science entitled "Seasonal variation in the positional distribution of fatty acids in bovine milk fat".